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  • 赌王平台appapp网址• 比二氯荧光黄 (DCFH) 有更高的特异性• 可动态监测活细胞内的过氧化氢• BES-H2O2-Ac 可穿透细胞膜,无需打孔• 极易溶于水,可制成水溶液使用• 可用于荧光显微镜、流式细胞仪

  • 赌王平台app平台官网 本品是迄今发现最适合用于生物内部深处活体成像的荧光素,发射光不会被血液、水分和黑色素吸收,有其他荧光素无法超越的灵敏度和穿透力,能透过血脑屏障,可用于皮下、肺部、脑部等器官的发光。AkaLumine-HCl是一种发光峰值在670-680nm处的荧光素类似物。

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The product is a serum-free medium designed for primary culture of rat and murine neurons, and is suited for culturing cells of the central nervous system. The product contains culture supernatant of rat glial cells.

 

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Neuron culture medium: 14 days

Conventional culture medium: approximately 1 month

 

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Neuron culture medium: 0.1 ×106 cells/mL

Conventional culture medium: 0.5~1.0×106 cells/mL

 

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Cells can be cultured with the culture medium alone, with no need for additional supplements.

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Number of cells: 0.1×106赌王平台app下载线路(isolated from the hippocampus of a mouse at embryonic days 18.5)
Seeding density: 500 μL/well(polylysine-coated glass bottom dish) 
Culture condition: Change half of the culture medium at days 3 and 7 (without the addition of Ara-C)

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Dr. Hirotaka James Okano and Mr. Yuki Ogawa, Division of Regenerative Medicine, Jikei University School of Medicine

▶The data suggested that neurons grown in our culture medium had a faster rate of dendrite outgrowth compared with neurons cultured in the competitor’s medium.

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Number of cells: 0.1×106赌王平台app网址平台(isolated from the hippocampus of a mouse at embryonic days 18.5)

Seeding density: 500 μL/well (polylysine-coated glass bottom dish)

Culture condition: Change half of the culture medium at days 3 and 7 (without the addition of Ara-C)

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Dr. Hirotaka James Okano and Mr. Yuki Ogawa, Division of Regenerative Medicine, Jikei University School of Medicine

▶The data suggested that neurons grown in our culture medium had a faster rate of dendrite outgrowth and were more viable compared with neurons cultured in the competitor’s medium.

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Number of cells: 0.1×106赌王平台app代理app (isolated from the hippocampus of a mouse at embryonic days 18.5)

Seeding density: 500 μL/well (polylysine-coated glass bottom dish)

Culture condition: Change half of the culture medium at days 3 and 7 (without the addition of Ara-C)

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Dr. Hirotaka James Okano and Mr. Yuki Ogawa, Division of Regenerative Medicine, Jikei University School of Medicine

▶The data revealed the presence of dendritic spines, which indicate maturity of neurons, on neurons cultured in our medium on day 14.

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Number of cells: 0.1×106赌王平台app地址二维码(isolated from the hippocampus of a mouse at embryonic days 18.5)

Seeding density: 500 μL/well (polylysine-coated glass bottom dish)

Culture condition: Change half of the culture medium at days 3 and 7 (without the addition of Ara-C)

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Dr. Hirotaka James Okano and Mr. Yuki Ogawa, Division of Regenerative Medicine, Jikei University School of Medicine

▶The data demonstrated that neurons cultured in our medium had multiple dendrites and one axon that are extended from the cell body, suggesting that they have properly matured.

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Number of cells: 0.1×106赌王平台app下载线路(isolated from the hippocampus of a mouse at embryonic days 18.5)

Seeding density: 500 μL/well (polylysine-coated glass bottom dish)

Culture condition: Final concentration at 1nM, supplemented with triiodothyronine

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Dr. Hirotaka James Okano and Mr. Yuki Ogawa, Division of Regenerative Medicine, Jikei University School of Medicine

▶Purkinje cells cultured in 1 nM of our culture medium supplemented with triiodothyronine stained positive for calbindin and had dendritic development.

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Suspension culture of cells after directed dopaminergic neuron differentiation at day 14 (42 days from directed differentiation)

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Dr. Hirotaka James Okano and Ms. Keiko Bouno, Division of Regenerative Medicine, Jikei University School of Medicine

▶The data demonstrated that iPS-derived neurons cultured in our medium stained positive for NeuN, suggesting that they are mature neurons.

 

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Suspension culture of cells after directed dopaminergic neuron differentiation at day 14 (42 days from directed differentiation)

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Dr. Hirotaka James Okano and Ms. Keiko Bouno, Division of Regenerative Medicine, Jikei University School of Medicine

▶The data demonstrated improved viability of TH-positive cells derived from directed dopaminergic neuron differentiation when maintained in our culture medium.

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Suspension culture of cells after directed dopaminergic neuron differentiation at day 14 (42 days from directed differentiation)

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Human iPS-derived neurons

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Human iPS-derived neurons

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Dr. Hirotaka James Okano and Ms. Keiko Bouno, Division of Regenerative Medicine, Jikei University School of Medicine

▶Ca2+ imaging revealed that some of the human iPS-derived neurons cultured in our medium had independent physiological functions.


We are committed to provide complete solutions

for life science applications enabling people

to make and deliver life improving discoveries.

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